Current Research in Nutrition and Food Science - An open access, peer reviewed international journal covering all aspects of Nutrition and Food Science

Identification of Single Nucleotide Polymorphisms in Selenium-Glutathione Peroxidase (GPX1) Gene

Ala A. Qatatsheh^1*, Rula Amr², Murad A. Al-Holy³, Sharaf Omar⁴, Amin N. Olaimat⁵, Ibrahim R. Darbour⁶

¹Department of Clinical Nutrition, College of Applied Health Sciences, Qassim University, Saudi Arabia and Department of Nursing, Princess Aisha Bint Al-Hussein Faculty of Nursing, Al-Hussein bin Talal University, Ma’an, Jordan.

²Department of Human Nutrition and Dietetics, American University of Madaba , Jordan, Madaba.

^3,4Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Science, The Hashemite University, Zarqa, Jordan.

⁵Department of Nutrition and Food Science Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt, Jordan.

⁶Department of Nutrition and Food Technology, Faculty of Agriculture, Mutah University, Karak, Jordan.

Corresponding Author Email: a.a.qatatsheh@gmail.com

Abstract:

Human genetic variation is quite common with single nucleotide polymorphisms (SNPs) accounting for the majority of the variants. In the present study, primers for amplification of the appropriate part of the human GPX1 gene by polymerase chain reaction (PCR) were designed. The aim of this study was to develop a restriction fragment length polymorphism (RFLP) assay to detect and characterize GPX1 polymorphism in the coding region and validate the assays by sequencing.
This study demonstrated that the RFLP method, which can be rapid, is reliable and valid as a tool for identifying the different polymorphisms with a high degree of specificity and sensitivity.

Keywords:

Glutathione Peroxidase; PCR; RFLP; Single Nucleotide Polymorphisms; Sequencing

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