Current Research in Nutrition and Food Science - An open access, peer reviewed international journal covering all aspects of Nutrition and Food Science

Inhibition of Calcium Oxalate Crystal Growth in Gel by Macrotyloma uniflorum, Phaseolus lunatus, and Phaseolus vulgaris

Salman Ahmed¹, Muhammad Mohtasheemul Hasan¹, Zafar Alam Mahmood², Jamelah Saleh Al-Otaibi³, Haroon Khan^4,5*

¹Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.

²Colorcon Limited – UK, Flagship House, Victory Way, Crossways, Dartford, Kent, DA QD-  England.

³Department of Chemistry, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia.

⁴Department of Pharmacy, Abdul Wali Khan University Mardan, Mardan, Pakistan.

⁵Department of Pharmacy, Korea University, Sejong, South Korea.

Corresponding Author E-mail:haroonkhan@korea.ac.kr

This study investigated the inhibitory effects of aqueous seed infusions from Macrotyloma uniflorum (MU), Phaseolus lunatus (PL), and Phaseolus vulgaris (PV) on calcium oxalate monohydrate (COM) crystal growth, a key factor in kidney stone formation. Infusions at concentrations of 5%, 10%, 15%, and 20% were tested using an in vitro gel technique with phytic acid (PA) as a control. After 40 days, SEM analysis revealed irregular, incompletely formed crystals in treated samples compared to well-formed prismatic structures in controls. Morphological changes included dumbbell shapes in PA-treated and prismatic or rosette forms in MU-treated crystals. EDS analysis showed reduced calcium content and increased carbon and oxygen levels, while FTIR confirmed significant functional group modifications, especially with MU. The inhibitory effect was dose-dependent, with MU showing the strongest inhibition, followed by PV and PL, suggesting MU’s potential as a natural preventive agent against calcium oxalate stone formation.

Antiurolithiatic activity; Calcium oxalate crystals; EDS; FTIR; SEM

[ HTML Full Text]