eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2019-12-25 7 3 819 827 10.12944/CRNFSJ.7.3.21 9627 article Identification of Single Nucleotide Polymorphisms in Selenium-Glutathione Peroxidase (GPX1) Gene Ala A. Qatatsheh 1 Rula Amr 2 Murad A. Al-Holy 3 Sharaf Omar 4 Amin N. Olaimat 5 Department of Nursing, Princess Aisha Bint Al-Hussein Faculty of Nursing and Health Sciences, Al-Hussein bin Talal University, Ma’an, Jordan and Department of Clinical Nutrition, Faculty of Applied Health Sciences, Qassim University, Arrass, Saudi Arabia Department of Human Nutrition and Dietetics, American University of Madaba , Jordan, Madaba Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Science, The Hashemite University, Zarqa, Jordan Department of Nutrition and Food Science Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt 19117, Jordan

Human genetic variation is quite common with single nucleotide polymorphisms (SNPs) accounting for the majority of the variants. In the present study, primers for amplification of the appropriate part of the human GPX1 gene by polymerase chain reaction (PCR) were designed. The aim of this study was to develop a restriction fragment length polymorphism (RFLP) assay to detect and characterize GPX1 polymorphism in the coding region and validate the assays by sequencing.
This study demonstrated that the RFLP method, which can be rapid, is reliable and valid as a tool for identifying the different polymorphisms with a high degree of specificity and sensitivity.

 https://www.foodandnutritionjournal.org/volume7number3/identification-of-single-nucleotide-polymorphisms-in-selenium-glutathione-peroxidase-gpx1-gene/ Glutathione Peroxidase PCR RFLP Single Nucleotide Polymorphisms Sequencing