eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2026-07-06 14 2 26503 article Herpandi Herpandi 1 Agusriansyah Saputra 1 Indah Widiastuti 1 Sabri Sudirman 1 Chalidazia 1 Department of Fisheries Product Technology, Universitas Sriwijaya, Indralaya, Indonesia This research investigates the extraction and characterization of nanopowder from snakehead fish (Channa striata) bones using ultrasonic treatment and variations in calcination temperatures. The fish bones were calcined at 500℃, 750℃, and 1000℃ for 5 hours to eliminate organic components and improve the crystallinity of the nanopowder. Ultrasonic waves were also employed to create cavitation conditions, promoting the formation of nanoparticles with reduced size. Characterization was performed using Scanning Electron Microscopy-Energy Dispersive X-ray Analysis (SEM-EDX), X-ray Diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FTIR). Five samples were analyzed: Sample A (untreated fish bone powder), Sample B (ultrasonic-treated), Sample X (ultrasonic-treated and calcined at 500℃), Sample Y (ultrasonic-treated and calcined at 750℃), and Sample Z (ultrasonic-treated and calcined at 1000℃). The results showed that increasing calcination temperature drove morphological transformation from heterogeneous platy fragments toward uniform spherical nanoparticles, while simultaneously enhancing calcium and phosphorus content and HAp crystallinity. Calcination at 1000°C yielded the optimal outcome, a mean particle size of 341 ± 197 nm, Ca and P concentrations of 31.02% and 18.25%, respectively, and crystallinity reaching 95%. These findings establish ultrasonic-assisted calcination at 1000°C as an effective and scalable route for producing high-crystallinity HAp nanopowder from an underutilized fishery by-product, with potential applications in bone tissue engineering and biomedical implants. https://www.foodandnutritionjournal.org/volume14number2/extraction-and-characterization-of-nanopowder-from-snakehead-fish-channa-striata-bones/ FTIR Nanopowder Snakehead fish bone SEM-EDX XRD