eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2026-08-17 14 2 26668 article Modou DIENG 1 Sékou KOUYATÉ 2 Awa NDIOUR 1 Fatou SENE 1 Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), Higher Polytechnic School (ESP), Cheikh Anta Diop University (UCAD), Dakar, Senegal. Higher Institute of Science and Veterinary Medicine (ISSMV), Dalaba, Republic of Guinea. Sachet-packaged water constitutes an important source of drinking water for urban populations, particularly in developing countries, due to the sometimes limited access to distributed potable water. The present study, conducted in December 2025, aimed to assess the microbiological quality of sachet-packaged water intended for human consumption in the city of Dakar (Senegal). A total of twenty-six (26) sachet water samples, supplied by producing companies as part of routine microbiological quality control, were analysed. The microbiological parameters investigated included total aerobic mesophilic flora (TAMF) at 22 °C, 30 °C, and 37 °C, faecal coliforms, total coliforms, and staphylococci. The conditions for transporting and storing the samples were followed. The analyses were performed using standardized microbiological methods. The results showed that 34.6% of the samples were contaminated with TAMF, sometimes with high microbial loads, while faecal and total coliforms were detected in 15.4% of the analysed samples. In contrast, no staphylococci were detected in any of the samples. These findings indicate that although the majority of the analysed sachet-packaged waters exhibited satisfactory microbiological quality, a non-negligible proportion remained non-compliant with recommended microbiological criteria, thereby constituting a potential risk to public health. The study highlights the need to strengthen sanitary control measures, ensure compliance with good hygiene practices, and implement regular monitoring of the microbiological quality of sachet-packaged water. https://www.foodandnutritionjournal.org/volume14number2/determination-of-the-level-of-microbiological-contamination-of-sachet-packaged-water-case-study-of-samples-received-at-the-laboratory-of-analysis-and-testing-of-the-ecole-superieure-polytechnique-de/ Coliforms Contamination Dakar Health Microbiology Staphylococci Water