eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2026-04-10 14 1 356 370 10.12944/CRNFSJ.14.1.24 25149 article Daisy Sharma 1 Pameena Narzary 1 Manash Pratim Sarma 2 Yahia Mazumdar 3 Ananya Kashyap 4 Programme of Food Nutrition and Dietetics, Assam down town University, Guwahati, India Programme of Biotechnology, Assam down town University, Guwahati, India Assam down town University, Guwahati, India Department of Home Science, HPB Girls College, Golaghat, India Musa balbisiana colla is a widely grown banana having medicinal properties. Having known it holds medicinal value. This present study was done to investigate the hepatoprotective effect of Musa balbasiana seeds and flower parts on hepatocytes damage and associated alteration of antioxidant enzymes in CCl4 model induced hepatotoxicity in experimental animals. Acute hepatotoxicity was induced by intraperitonially on 11th and 12th day of the experiment Adult male Sprague Dawley (SD) rats with body weight between 200 g and 300 g were selected for the study and extracts at dose of 200mg and 400mg seed and flower were administered orally for 14 consecutive days. Hepatotoxicity was determined after 14days of CCl4 administration. The activities of various serum biomarkers aspartate aminotransferase (AST) activity, alanine aminotransferase (ALT) activity, alkaline phosphatase (ALP) activity and lactate dehydrogenase (LDH) activity, levels of enzymatic and non-enzymatic antioxidant (GSH, GST, catalase, Vitamin C, Nitrites) and lipid peroxidation level were estimated in all the rats. Our study showed decrease in all enzymatic and non-enzymatic antioxidant level and a marked increase level of lipid peroxidation in the entire CCl4 induced group compared to normal group. Supplementation of MB seed and flower for 14 days restored the antioxidant levels as well as malondialdehyde level. Increasing the dose i.e 400mg in both seeds and flower extract showed effective activities compared to 200mg administration. Also the biochemical investigations were further supported by histopathological examination. Our results suggested that the MB (Musa balbisiana seeds extract) 400 mg/kg has a significant therapeutic benefit compared to MB flower against the hepatotoxic complications by attenuating oxidative stress and lipid peroxidation. Thus MB seed can have efficacy role as hepatoprotective agent in CCl4 induced liver pathology. https://www.foodandnutritionjournal.org/volume14number1/in-vivo-efficacy-study-on-musa-balbasiana-seeds-and-inflorescence-for-its-hepatoprotective-effect/ Antioxidant Enzymes Flower Hepatoprotective Musa balbisiana colla Seed Serum level