eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2026-04-10 14 1 221 229 10.12944/CRNFSJ.14.1.14 25437 article Kyoung-Sun Seo 1 Kyeong Won Yun 2 Department of Food Science and Technology, Jangheung Research Institute for Mushroom Industry, Jangheung, Republic of Korea. Department of Bio-Oriental Medicine Resources, Sunchon National University, Suncheon, Republic of Korea. The flower bud of Magnolia denudata has been used as a traditional medicine to treat rhinitis and gastrointestinal disorder, but the flower has not been used for medicine in Korea. This study evaluated the extracts of Magnolia denudata and Magnolia kobus flower and flower bud for antimicrobial and antioxidant properties. The MIC by disk diffusion assay was used for the antimicrobial activity against three Gram-positive bacteria, four Gram-negative bacteria strains and one yeast. Antioxidant activity for three solvent extracts (hot water, ethanol 70%, ethanol 100%) of the two Magnolia plants was evaluated by using EDA and ABTS radical scavenging activity. The water fraction of the two Magnolia flower bud was shown to have the highest antimicrobial activity, with the MIC value of 5.0 mg/ml against B. cereus and S. typhimurium. The hot water extract of Magnolia denudata (flower bud) showed the highest antioxidant activity, with the lowest IC₅₀ values of 301.82±7.92 µg/mL in the EDA assay and 219.48±6.32 µg/mL in the ABTS assay. In addition, the hot water extract of the four samples revealed the highest TPC and TFC as compared to the other extracts and the order was flower bud of M. denudata > flower of M. denudata > flower of M. kobus > flower bud of M. kobus. There appears to be a relationship between the TPC (+TFC) and the antioxidant properties of Magnolia denudata and Magnolia kobus. This is first report on in vitro antimicrobial and antioxidant properties of the two Magnolia plants, these data imply that the two Magnolia plants might to be useful for future development in pharmaceutical, food and cosmetic industries. https://www.foodandnutritionjournal.org/volume14number1/in-vitro-antimicrobial-and-antioxidant-properties-of-extracts-from-magnolia-denudata-and-magnolia-kobus-flower-bud-and-flower/ Antimicrobial Antioxidant Flower Flower Bud Magnolia denudata Magnolia kobus