eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2026-04-10 14 1 194 203 10.12944/CRNFSJ.14.1.12 25631 article Annisa Khaira Ma’adi 1 Nina Resti 1 Kis Djamiatun 3 Ahmad Syauqy 1 Gemala Anjani 1 Diana Nur Afifah 1 Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia Doctoral Study Program of Medical and Health Sciences, Universitas Diponegoro, Semarang, Indonesia Department of Parasitology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia Laboratory of Sustainable Diets and Biodiversity, Integrated Laboratory for Research and Services, Universitas Diponegoro, Semarang, Indonesia This study aimed to evaluate the effect of sorghum tempeh (Sorghum bicolor (L.) Moench cv. Numbu) intervention on hs-CRP (high-sensitive C-Reactive Protein) levels and SCFA (Short-Chain Fatty Acid) concentrations in the cecum of rats given a diet high in atherogens. There were five groups of male Wistar rats: three intervention groups, a negative control, and a normal control receiving sorghum-based diets containing sorghum flour (P1), sorghum tempeh 5.09 g/mL (P2), and sorghum tempeh 7.63 g/mL (P3). The results showed that hs-CRP levels in the P3 group decreased significantly from 17.06 ± 0.58 mg/L to 4.11 ± 0.34 mg/L, representing an approximate 75.9% reduction compared to baseline values (p<0.05). Among SCFAs, the P3 group had the highest acetate concentration (7.18 ± 0.25 µmol/g), while the highest butyrate concentration was observed in P2 (2.44 ± 0.19 µmol/g). Propionate concentrations were not significantly different among groups. These findings suggest that sorghum tempeh, particularly at moderate to higher doses, can reduce inflammatory responses and modulate SCFA production in rats under an atherogenic diet. However, conclusions regarding atherosclerosis improvement remain preliminary since histopathological or endothelial biomarker analyses were not performed. https://www.foodandnutritionjournal.org/volume14number1/effect-of-giving-sorghum-tempeh-on-high-sensitive-c-reactive-protein-hs-crp-levels-and-short-chain-fatty-acid-scfa-concentrations-in-the-cecum-of-rats-induced-by-an-atherogenic-diet/ Atherogenic Diet Cecum of Rats hs-CRP SCFA Sorghum Tempeh