eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2025-11-20 13 3 1394 1408 10.12944/CRNFSJ.13.3.27 24409 article Sabrine Jasi 1 Wissem Mnif 3 Jose Antonio Mendiola 4 Anis Ben Hsouna 5 Zaina Algarni 7 Miroslava Kačániová 8 Laboratory of Biotechnology and Valorisation of Bio-GeoResources, University of Manouba, Ariana, Tunisia Faculty of Sciences of Bizerte, University of Carthage, Bizerte, Tunisia Department of Chemistry, College of Science, University of Bisha, Bisha, Saudi Arabia Foodomics Laboratory, Institute of Food Science Research (CIAL-CSIC), Madrid, Spain Laboratory of Biotechnology and Plant Improvement, Centre of Biotechnology of Sfax, Sfax, Tunisia 6. Department of Environmental Sciences and Nutrition, University of Monastir, Monastir, Tunisia The Phoenix dactylifera, a tropical and subtropical tree of the Palmae family (Arecaceae), commonly known as the date palm, holds the distinction of being one of the earliest crops cultivated by man. Date palms come in several varieties due to the fact that are cultivated in different places with different Ecologic environment characteristics in especially North Africa and the Middle East. In this work the optimum extraction conditions of the phytochemicals compounds from the edible part of the fruit and the seeds between those varieties using sub and supercritical fluids and allow their characterisation and to determine their antioxidant activities and composition. In this sense the date kernel oil obtained by supercritical CO₂ yielded up to 9% with a fatty acid composition similar to olive oil, which demonstrates a good way for valorization of date by-product. Meanwhile, date flesh extract provided high antioxidant activity associated to phenolic compounds, especially using high temperatures (150 ºC) and pressurized ethanol for the extraction. https://www.foodandnutritionjournal.org/volume13number3/supercritical-and-subcritical-extraction-of-date-fruits-phoenix-dactylifera-l-and-their-phytochemical-composition/ Antioxidant activity Supercritical CO₂ extraction Phytochemicals Phoenix dactylifera