eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2025-08-25 13 2 861 875 10.12944/CRNFSJ.13.2.22 23277 article Farah Nadiah Mohd Fazil 1 Saiful Irwan Zubairi 1 Zalifah Mohd Kasim 1 Roslee Rajikan 2 Isa Naina Mohamed 3 Ikhwan Zakaria 5 Department of Food Sciences, Faculty of Science and Technology, UKM Bangi, Selangor Darul Ehsan, Malaysia Department of Dietetics, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur Department of Pharmacology, Faculty of Medicine, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur. Malaysia UNIPEQ, Block A, UKM-MTDC Technology Centre, Universiti Kebangsaan Malaysia Bangi, Selangor, Malaysia Pusat PERMATA@Pintar Negara, Universiti Kebangsaan Malaysia, UKM Bangi, Selangor Darul Ehsan, Malaysia Kaempferia angustifolia, a notable medicinal herb within the ginger family, comprises around 60 species of rhizomatous plants. Commonly referred to as temu kunci, kunci pepet, or Thao Nhang Haeng in Southeast Asia, it is renowned for its aromatic rhizomes, traditionally utilized to address ailments like colds, digestive issues, and fevers. Recent research underscores its potential in cancer chemoprevention, alongside antioxidants, antimicrobial, anti-obesity, and anti-allergic properties. This study focuses on four key flavonoids found in the innovative FemiVine V jelly product and the raw extract of the Kaempferia angustifolia plant. The jelly exhibited a 60% higher total phenolic content (31.93 ± 0.20 mg/g) compared to the raw extract (12.0 ± 0.23 mg/g, p<0.05). Antioxidant assays revealed superior DPPH radical scavenging activity in the jelly (67.6 ± 0.15%) versus the extract (30.54 ± 0.10%, p<0.05), supported by FRAP values of 5.08 ± 0.01 mg/100 g (jelly) and 4.81 ± 0.05 mg/100 ml (extract). Cytotoxic screening against MDA-MB-231 breast cancer cells showed stronger activity in the raw extract (IC₅₀: 82 ± 0.06 mg/ml) compared to the jelly (IC₅₀: 125 ± 0.20 mg/ml, p<0.05). In-silico analysis highlighted vitexin and β-sitosterol as promising candidates for peripheral cancer treatment due to favorable pharmacokinetic properties, including gastrointestinal absorption and lack of brain penetration. Further research is needed to assess their selective cytotoxicity against cancer cells versus normal fibroblasts. https://www.foodandnutritionjournal.org/volume13number2/bioactive-potential-and-in-silico-insights-of-flavonoid-enriched-femivine-v-jelly-and-kaempferia-angustifolia-extracts-in-antioxidant-and-anticancer-applications/ Antioxidant activity Cancer chemoprevention Flavonoids In silico testing Kaempferia angustifolia