eng Enviro Research Publishers Current Research in Nutrition and Food Science Journal 2347-467X 2322-0007 2016-10-25 4 Special Issue Nutrition in Conference October 2016 77 86 10.12944/CRNFSJ.4.Special-Issue-October.10 2946 article Effect of in Vitro Gastrointestinal Digestion on Bioactivity of Poultry Protein Hydrolysate Torkova Anna 1 Kononikhin Alexey 2,3 Bugrova Anna 3 Khotchenkov Vyacheslav 1 Tsentalovich Mikhail 1 Medvedeva Ulia 4 Federal Research Centre “Fundamentals of Biotechnology” of The Russian Academy of Sciences”, 119071, Russia, Moscow, Leninskiy Prospekt, 33, bld. 2 Moscow Institute of Physics and Technology, Moscow, Russia; Dolgoprudnyi, 141700 Moscow Region, Russia. Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, 119334, Kosygina street, 4, Moscow, Russia. Institute of General Pathology and Pathophysiology, 125315, Baltiysaya street, 8, Moscow, Russia.

In vitro simulated gastrointestinal digestion (GID) was performed to evaluate changes in bioactive properties of Poultry protein hydrolysate HCP Premium P150 (PPH) showing strong antioxidant (448.2±37.0 µM TE/g of protein) and moderate Angiotensin-I converting enzyme inhibitory activity (IC50 0.617±0.022 mg/ml). Antioxidant and ACE-inhibitory activity were measured with use of ORAC assay and FRET-substrate methods, correspondingly. Gastric digestion (GD) increased ACE inhibitory activity 2.23 times and didn’t change antioxidant activity of PPH significantly. The subsequent intestinal digestion increased antioxidant activity 1.29 times and didn’t change ACE-inhibitory activity significantly.  New potent ACE-inhibitory peptides: APGAPGPVG (IC50 16.2±3.8 µM), PDLVF (IC50 84.9±6.3 µM) and antioxidant dipeptide WG (2.29±0.04 µM TE/µM) were identified in the digested PPH. The digested PPH proved to be a rich source of antioxidant and ACE inhibiting molecules and could be a potential new food ingredient used for prevention or treatment of socially significant diseases.

 https://www.foodandnutritionjournal.org/vol04nospl-issue-conf-october-2016/effect-of-in-vitro-gastrointestinal-digestion-on-bioactivity-of-poultry-protein-hydrolysate/ Antioxidant hydrolysate ACE-inhibitory hydrolysate in vitro gastrointestinal digestion chicken hydrolysate