Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry. Besides these known properties of probiotics, in recent years many researchers have focused on their anti-genotoxic called DNA-bioprotective effects.  Human gets exposure with genotoxins such as mycotoxins, polycyclic aromatic hydrocarbons, and n-nitroso-compounds through diet and environmental contaminations. Aflatoxin B₁ is mycotoxin which was reported one of the most potent hepato-carcinogen and its exposure stems from human diet. In this study, we aimed to investigate DNA-bioprotective effect of Lactobacillus plantarum on human colon adenocarcinoma (Caco-2) cells against Aflatoxin B₁ (AFB₁) with comet assay without metabolic activation. The results showed that DNA-bioprotective effect of L. plantarum did reduce the AFB₁’s genotoxic effect on colon adenocarcinoma (Caco-2) cells. Positive control (50 μM H₂O₂ applied) and high dose (>10ppm) AFB₁ applied cells have the same comet tail appearance. Only visual scoring is performed.  Besides negative control cells (Only PBS) and Lactobacillus plantarum+AFB1 mixture showed the same manner with each other; no comet tail detected. These results clearly indicate that L. plantarum is capable of reduce AFB₁ safely without producing any by-products.