Effect of in Vitro Gastrointestinal Digestion on Bioactivity of Poultry Protein Hydrolysate

Torkova Anna¹, Kononikhin Alexey^2,3, Bugrova Anna³, Khotchenkov Vyacheslav¹, Tsentalovich Mikhail*¹ and Medvedeva Ulia⁴

¹Federal Research Centre “Fundamentals of Biotechnology” of The Russian Academy of Sciences”, 119071, Russia, Moscow, Leninskiy Prospekt, 33, bld. 2
²Moscow Institute of Physics and Technology, Moscow, Russia; Dolgoprudnyi, 141700 Moscow Region, Russia
³Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, 119334, Kosygina street, 4, Moscow, Russia
⁴Institute of General Pathology and Pathophysiology, 125315, Baltiysaya street, 8, Moscow, Russia.

Corresponding Author Email: snowsurfers1@gmail.com

In vitro simulated gastrointestinal digestion (GID) was performed to evaluate changes in bioactive properties of Poultry protein hydrolysate HCP Premium P150 (PPH) showing strong antioxidant (448.2±37.0 µM TE/g of protein) and moderate Angiotensin-I converting enzyme inhibitory activity (IC₅₀ 0.617±0.022 mg/ml). Antioxidant and ACE-inhibitory activity were measured with use of ORAC assay and FRET-substrate methods, correspondingly. Gastric digestion (GD) increased ACE inhibitory activity 2.23 times and didn’t change antioxidant activity of PPH significantly. The subsequent intestinal digestion increased antioxidant activity 1.29 times and didn’t change ACE-inhibitory activity significantly.  New potent ACE-inhibitory peptides: APGAPGPVG (IC₅₀ 16.2±3.8 µM), PDLVF (IC₅₀ 84.9±6.3 µM) and antioxidant dipeptide WG (2.29±0.04 µM TE/µM) were identified in the digested PPH. The digested PPH proved to be a rich source of antioxidant and ACE inhibiting molecules and could be a potential new food ingredient used for prevention or treatment of socially significant diseases.

Antioxidant hydrolysate; ACE-inhibitory hydrolysate; in vitro gastrointestinal digestion; chicken hydrolysate

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